Review of herbicide label requirements: Enlist One and Enlist Duo

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This article is the third in a series reviewing unique or updated label requirements for key herbicides. Instructions printed on your herbicide label supersede this information.

The Enlist herbicide resistance trait is available in certain transgenic (GMO) varieties of three crops: corn, cotton, and soybean. Enlist corn is tolerant of 2,4-D, glyphosate, and ‘fop’ graminicides; Enlist cotton is tolerant of 2,4-D, glyphosate, and glufosinate (Liberty, etc.); and Enlist E3 soybeans are tolerant of 2,4-D, glyphosate, and glufosinate. However, the only 2,4-D formulations labeled for use in-crop are Enlist One (2,4-D) and Enlist Duo (2,4-D plus glyphosate). These products contain 2,4-D choline, which is intended to reduce off-target movement of the herbicide. Similarly, Assure II is the only graminicide labeled for use in Enlist corn.

Some of the unique label requirements to be aware of when using Enlist One or Enlist Duo are listed below.

  • Apply between emergence and V8 or 30 inches (whichever happens first) in corn or R2 (full flowering) in soybean. In cotton, the application window is emergence through mid-bloom.
  • Use only specified tank-mix partners found at
  • Use only approved nozzles, found at
  • Only spray when wind speed is less than 15 mph and when wind direction is away from sensitive crops.
  • Maintain a 30-foot downwind buffer between treated area and sensitive areas.
  • Do not spray in temperature inversions. Monitor temperature inversions using the Kansas Mesonet Inversion tool:
  • Use 10-15 GPA of water – do not use liquid N as a carrier.
  • Clean sprayer thoroughly after application.

In addition, some growers last summer experienced tank-mix compatibility issues when mixing Enlist One with some glyphosate formulations. As a result, Corteva is recommending at least 5 minutes of agitation after adding any AMS product and 2-3 minutes between other products.



Sarah Lancaster, Extension Weed Science Specialist

Tags:  drift label requirements Enlist herbicides